%A Fukutomi Y
%A Matsuoka M
%A Minagawa F
%A Toratani S
%A Mccormick G
%A Krahenbuhl J
%T IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae
%0 Journal Article
%D 2004
%J International Journal of Leprosy and other Mycobacterial Diseases
%P 0148-916X
%V 72
%N 1
%X In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MΦ). Supplemental IL10, but not TGFβ, bolstered, directly or indirectly, M. leprae metabolism in mouse MΦ. In the cell culture system temperature setting is extremely important and 31 to 33°C incubation temperature was more permissive than 37°C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.