%A Ishaque M
%A Togola D
%A Sticht-Groh V
%T α-Ketoglutarate dehydrogenase in the in vitro-grown Mycobacterium lepraemurium
%0 Journal Article
%D 1994
%J International Journal of Leprosy and other Mycobacterial Diseases
%P 0148-916X
%V 62
%N 3
%X The Hawaiian and Kumato strains of Mycobacterium lepraemurium were cultivated on Ogawa egg-yolk medium, and the α-ketoglutarate dehydrogenase activity was investigated in cell-free preparations of this mycobacterium. The enzymatic activity was mainly localized in the particulate fraction (150,000 × g pellet), and extremely low activity was found in the soluble fraction (150,000 × g supernatant). a-Ketoglutarate dehydrogenase was not stable; the activity was lost completely when the enzyme was kept at 45ºC for 1 hr or stored at -70ºC. The enzyme reduced only NAD+ but not NADP+ by α-ketoglutarate, indicating the presence of NAD+-dependent a-ketoglutarate dehydrogenase in cultivated M. lepraemurium.