%A Ishaque M %A Togola D %A Sticht-Groh V %T α-Ketoglutarate dehydrogenase in the in vitro-grown Mycobacterium lepraemurium %0 Journal Article %D 1994 %J International Journal of Leprosy and other Mycobacterial Diseases %P 0148-916X %V 62 %N 3 %X The Hawaiian and Kumato strains of Mycobacterium lepraemurium were cultivated on Ogawa egg-yolk medium, and the α-ketoglutarate dehydrogenase activity was investigated in cell-free preparations of this mycobacterium. The enzymatic activity was mainly localized in the particulate fraction (150,000 × g pellet), and extremely low activity was found in the soluble fraction (150,000 × g supernatant). a-Ketoglutarate dehydrogenase was not stable; the activity was lost completely when the enzyme was kept at 45ºC for 1 hr or stored at -70ºC. The enzyme reduced only NAD+ but not NADP+ by α-ketoglutarate, indicating the presence of NAD+-dependent a-ketoglutarate dehydrogenase in cultivated M. lepraemurium.